Experimental model of nephropathy associated with diabetes mellitus in mice

Purpose: Nontransmissible chronic diseases, such as diabetes mellitus (DM) and nephropathy, affect a significant portion of the population, often treated due to injuries that require healing and regeneration. To create an experimental model of associated comorbidities, for healing and regeneration studies, protocols for induction of nephropathy by ischemia and reperfusion (I/R) and induction of DM by injection of streptozotocin (STZ) were associated. Methods: Sixty-four mice (Mus musculus), female, adult, Swiss strain, weighing approximately 20 g, were divided into four groups: G1: control (n = 24), G2: nephropathy group (N) (n = 7), G3, DM (n = 9), and G4: N+DM (n = 24). Arteriovenous stenosis (I/R) of the left kidney was performed as the first protocol. The animals received a hyperlipidemic diet for 7 days after the injection of STZ (150 mg/kg, via i.p.) and an aqueous glucose solution (10%) for 24 h. The animals in the G3 and G4 groups were observed for 14 days before receiving the diet and STZ. The evolution of nephropathy was observed using a urine test strip and the DM, through the analysis of blood glucose with a reagent strip on a digital monitor. Results: The ischemic induction protocols of nephropathy and DM with STZ, associated, were sustainable, low-cost, and without deaths. There were alterations compatible with initial renal alterations, in the first 14 days, such as increased urinary density, pH alteration, presence of glucose, proteins and leukocytes, when compared to the control group. DM was confirmed by the presence of hyperglycemia 7 days after induction and its evolution after 14 days. The animals in the G4 group showed constant weight loss when compared to the other groups. It was possible to observe morphological alterations in the kidneys submitted to I/R, regarding coloration, during surgery and after the end of the observation period, in the volume and size of the left kidney, when compared to the contralateral kidney. Conclusion: It was possible to induce nephropathy and DM associated in the same animal, in a simple way, confirmed with rapid tests, without losses, providing a basis for future studies.

O uso do laser de baixa potência por enfermeiro no tratamento de lesões cutâneas e orais / The use of low power laser by nurses in the treatment of cutaneous and oral lesions / El uso del láser de baja potencia por enfermero en el tratamiento de lesiones cutáneas y orales

Objetivo: Analisar a produção científica nacional e internacional acerca da utilização da laserterapia pelo enfermeiro no tratamento de lesões cutâneas e orais. Métodos: Tratou-se de uma revisão integrativa realizada nas bases de dados SciELO, Lilacs, Pubmed e Bdenf, nos últimos sete anos, utilizando os seguintes descritores: laser, feridas, lesões por pressão, mucosite oral, fissuras mamárias e enfermagem. Resultados: Não foram localizados artigos científicos nas bases de dados pesquisadas, utilizando os descritores laser, mucosite oral, fissuras mamárias e enfermagem. Foram localizados 83 artigos científicos e, selecionados 4, que se adequaram à temática laserterapia, feridas, lesões por pressão e enfermagem. Conclusão: Embora o Conselho Federal de Enfermagem tenha aprovado a utilização da laserterpia para tratamento de lesões cutâneas e orais, enfatiza- se a necessidade dos enfermeiros se capacitarem em laserterapia, visando ao tratamento de lesões, pois o laser de baixa potência representa um tratamento promissor para acelerar o reparo tecidual.(AU)

Objective: To analyze the national and international scientific production about the use of laser therapy by the nurse in the treatment of cutaneous and oral lesions. Methods: An integrative review of SciELO, Lilacs, Pubmed and Bdenf databases was performed in the last seven years using the following descriptors: laser, wounds, pressure lesions, oral mucositis, breast fissures and nursing. Results: No scientific articles were found in the databases searched, using the laser descriptors, oral mucositis, breast fissures and nursing. A total of 83 scientific articles were selected, and 4 selected, which were adapted to the topic of laser therapy, wounds, pressure lesions and nursing. Conclusion: Although the Federal Nursing Council approved the use of lasertherapy for the treatment of cutaneous and oral lesions, it is emphasized the need of nurses to be trained in laser therapy, aiming at the treatment of injuries, since the low power laser represents a promising treatment to accelerate tissue repair.(AU)

Objetivo: Analizar la producción científica nacional e internacional acerca de la utilización de la láserterapia por el enfermero en el tratamiento de lesiones cutáneas y orales. En los últimos siete años, se utilizaron los siguientes descriptores: láser, heridas, lesiones por presión, mucositis oral, fisuras mamarias y enfermería. Métodos: Se trata de una revisión integrativa realizada en las bases de datos SciELO, Lilacs, Pubmed y Bdenf. Resultados: No se localizaron artículos científicos en las bases de datos investigadas, utilizando los descriptores láser, mucositis oral, fisuras mamarias y enfermería. Se localizaron 83 artículos científicos y, seleccionados 4, que se adecuaron a la temática láserterapia, heridas, lesiones por presión y enfermería. Conclusion: Aunque el Consejo Federal de Enfermería ha aprobado la utilización de la láserterpia para el tratamiento de lesiones cutáneas y orales, se enfatiza la necesidad de que los enfermeros se capaciten en láserterapia, buscando el tratamiento de lesiones, pues el láser de baja potencia representa un tratamiento prometedor para acelerar la reparación del tejido.(AU)